雌激素受體ERβ配體結合區的異源表達與純化

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雌激素受體ERβ配體結合區的異源表達與純化

時國慶, 郭彥峰, 魏巍, 宋青, 張懷, 鐘廣蓉

文章導航 > 工程科學學報 > 2007 > 29(S2): 208-211

時國慶, 郭彥峰, 魏巍, 宋青, 張懷, 鐘廣蓉. 雌激素受體ERβ配體結合區的異源表達與純化[J]. 工程科學學報, 2007, 29(S2): 208-211. doi: 10.13374/j.issn1001-053x.2007.s2.087

引用本文:

時國慶, 郭彥峰, 魏巍, 宋青, 張懷, 鐘廣蓉. 雌激素受體ERβ配體結合區的異源表達與純化[J]. 工程科學學報, 2007, 29(S2): 208-211. doi: 10.13374/j.issn1001-053x.2007.s2.087

SHI Guoqing, GUO Yanfeng, WEI Wei, SONG Qing, ZHANG Huai, ZHONG Guangrong. Heterologous expression and purification of the ligand binding domain of estrogen receptor β[J]. Chinese Journal of Engineering, 2007, 29(S2): 208-211. doi: 10.13374/j.issn1001-053x.2007.s2.087

Citation:

SHI Guoqing, GUO Yanfeng, WEI Wei, SONG Qing, ZHANG Huai, ZHONG Guangrong. Heterologous expression and purification of the ligand binding domain of estrogen receptor β[J]. Chinese Journal of Engineering, 2007, 29(S2): 208-211. doi: 10.13374/j.issn1001-053x.2007.s2.087

時國慶, 郭彥峰, 魏巍, 宋青, 張懷, 鐘廣蓉. 雌激素受體ERβ配體結合區的異源表達與純化[J]. 工程科學學報, 2007, 29(S2): 208-211. doi: 10.13374/j.issn1001-053x.2007.s2.087

引用本文:

時國慶, 郭彥峰, 魏巍, 宋青, 張懷, 鐘廣蓉. 雌激素受體ERβ配體結合區的異源表達與純化[J]. 工程科學學報, 2007, 29(S2): 208-211. doi: 10.13374/j.issn1001-053x.2007.s2.087

SHI Guoqing, GUO Yanfeng, WEI Wei, SONG Qing, ZHANG Huai, ZHONG Guangrong. Heterologous expression and purification of the ligand binding domain of estrogen receptor β[J]. Chinese Journal of Engineering, 2007, 29(S2): 208-211. doi: 10.13374/j.issn1001-053x.2007.s2.087

Citation:

SHI Guoqing, GUO Yanfeng, WEI Wei, SONG Qing, ZHANG Huai, ZHONG Guangrong. Heterologous expression and purification of the ligand binding domain of estrogen receptor β[J]. Chinese Journal of Engineering, 2007, 29(S2): 208-211. doi: 10.13374/j.issn1001-053x.2007.s2.087

雌激素受體ERβ配體結合區的異源表達與純化

doi: 10.13374/j.issn1001-053x.2007.s2.087

北京科技大學應用科學學院, 北京 100083

基金項目:

國家自然科學基金資助項目(No.20507002)

作者簡介:
時國慶(1972-),男,副教授

中圖分類號: Q591.1⁺1
計量
- 文章訪問數: 188
- HTML全文瀏覽量: 66
- PDF下載量: 4
- 被引次數: 0
出版歷程
- 收稿日期: 2007-08-15
- 網絡出版日期: 2021-08-16

Heterologous expression and purification of the ligand binding domain of estrogen receptor β

Applied Science School, University of Science and Technology Beijing, Beijing 100083, China

摘要: 為研究環境化合物與雌激素受體的相互作用,在大腸桿菌中異源表達了人雌激素受體ERβ的配體結合區(hERβ-LBD)蛋白。在誘導劑(IPTG)濃度為0.2mmol·L^-1,培養溫度25℃,誘導時間為8h的優化表達條件下,經親和色譜法純化后,hERβ(LBD蛋白的產量可達236mg·L^-1.
- 雌激素受體 /
- 配體結合區 /
- 表達 /
- 純化
Abstract: In order to investigate the interaction between environmental chemicals and estrogen receptors,the ligand binding domain of human estrogen receptor β(hERβ-LBD) was expressed in Escherichia coli.Based on the optimized expression conditions(IPTG,0.2 mmol·L^-1;culture temperature,25℃;time of induction,8 h),after purified by affinity chromatography,the production of hERβ-LBD can be reached to 236 mg·L^-1.
- estrogen receptor /
- ligand binding domain /
- expression /
- purification

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文章訪問數: 188
HTML全文瀏覽量: 66
PDF下載量: 4
被引次數: 0

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