小球藻USTB01的異養培養和葉黃素的生產

王素琴; 李雅雯; 閆海; 楊帥; 林海

doi:10.13374/j.issn1001-053x.2007.08.003

小球藻USTB01的異養培養和葉黃素的生產

doi: 10.13374/j.issn1001-053x.2007.08.003

王素琴^1,2,
李雅雯³,
閆海¹,
楊帥²,
林海²

1. 北京科技大學應用科學學院, 北京 100083;
2. 北京科技大學土木與環境工程學院, 北京 100083;
3. 北京航空航天大學生物工程系, 北京 100083

基金項目:

北京科技大學大學生創新基金資助項目

國家自然科學基金資助項目(No.20377047)

詳細信息

作者簡介:
王素琴(1982-),女,碩士研究生;閆海(1962-),男,教授

中圖分類號: Q914.82
計量
- 文章訪問數: 181
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- PDF下載量: 6
- 被引次數: 0
出版歷程
- 收稿日期: 2006-02-27
- 修回日期: 2006-12-07
- 網絡出版日期: 2021-08-16

Heterotrophic culture of Chlorella sp USTB01 and production of lutein

1. Applied Science School, University of Science and Technology Beijing, Beijing 100083, China;
2. Civil and Environmental Engineering School, University of Science and Technology Beijing, Beijing 100083, China;
3. Department of Biological Technology, Beijing University of Aeronautics and Astronautics, Beijing 100083, China

摘要

摘要: 研究了異養無光照條件下不同碳源、氮源和碳氮質量比對小球藻生長及葉黃素產生的影響.結果表明,葡萄糖和硝酸鉀分別是支持小球藻USTB01持續快速生長的最佳碳源和氮源.以葡萄糖和硝酸鉀分別作為唯一碳源和氮源時,在初始氮質量濃度都為0.28g·L^-1情況下,碳氮質量比為25:1是促進小球藻生長的優化控制條件.硝酸鉀是促進小球藻USTB01葉黃素生物合成的最佳氮源,但在碳氮質量比從15到30的范圍內,小球藻細胞中葉黃素含量隨碳氮質量比的升高而降低.
- 小球藻 /
- 異養培養 /
- 葉黃素 /
- 碳源 /
- 氮源 /
- 碳氮比
Abstract: The effects of carbon and nitrogen sources as well as carbon-to-nitrogen mass ratio on the growth of Chlorella sp USTB01 and the production of lutein were investigated under the condition of heterotrophic culture in.dark. The results show that glucose and potassium nitrate were the best carbon and nitrogen sources to support the rapid and continuous growth of Chlorella sp USTB01 respectively. When glucose and potassium nitrate were used as the sole carbon and nitrogen sources and the initial concentration of nitrogen was 0.28 g·L^-1, the growth of Chlorella sp increased with the increase of carbon-to-nitrogen mass ratio in the range from 15 to 25. Potassium nitrate was the best nitrogen source to promote biosynthesis of lutein by Chlorella sp USTB01 and the content of lutein in the cells of Chlorella sp USTB01 declined with the increase of carbon-nitrogen mass ratio from 15 to 30.
- Chlorella sp /
- heterotrophic culture /
- lutein /
- carbon source /
- nitrogen source /
- carbon-to-nitrogen ratio