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降解高含鹽溴氨酸菌種的選育與鑒定

Breeding and identification of bacteria for high-salt bromamine acid

  • 摘要: 從生物接觸氧化池的生物膜中篩選分離出10株在高鹽條件下對蒽醌染料中間體溴氨酸有降解脫色作用的菌株.通過對10株菌株的逐級馴化復篩,得到一株脫色效果好且脫色能力穩定的菌株C-7,經鑒定為弗氏檸檬酸桿菌(Citrobacter freundil).菌株C-7的耐鹽性試驗結果表明,鹽的質量分數為1%~3%時,對菌株的生長和脫色沒有影響,脫色率為80%以上;鹽的質量分數為7%時,菌株C-7也具有較高的耐受力,脫色率可達到50%以上;鹽的質量分數為10%時,對菌株的生長產生抑制作用,不能降解溴氨酸.菌株最適生長條件為:蛋白胨7g·L-1,pH7.0,搖床轉速160r·min-1,250mL搖瓶中裝液量120mL,接種量10%,在此條件下,在鹽的質量分數為1%、蛋白胨為唯一氮源的培養基中,溴氨酸(100mg·mL-1)的脫色率可達85%以上.

     

    Abstract: 10 strains,capable of degrading and decolorizing bromamine acid under high salt conditions,which is an intermediate of anthraquinone dye,were isolated from the biomembrane of a bio-contact oxidation tank.A strain named C-7 which has good decolorizing effect and stable decolorization ability was obtained by acclimating the 10 strains.It was identified as Citrobacter freundil.Salt tolerance test results show that the strain C-7 can grow with a decolorization rate of more than 80% when the mass fraction of NaCl is 1% to 3%,and NaCl has no effect on the growth and decolorization of the strain.When the mass fraction of NaCl is 7%,C-7 show high tolerance and the decolorization rate is more than 50%.NaCl can inhibit the growth of the strain C-7 and bromamine acid cannot be degraded when the mass fraction increases to 10%.Under the optional growth conditions of pH 7.0,the rotating rate of 160r·min-1,the liquid volume of 120mL in a 250mL flask,inocula of 10% and peptone of 7g·L-1 as the only nitrogen source,when the mass fraction of NaCl is 1%,the decolorization rate of bromamine acid(100mg·L-1) can reach to 85% or higher.

     

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