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水源水中2-MIB降解菌的篩選

Isolation of a bacterium capable of removing 2-methylisoborneol from water

  • 摘要: 針對特定水處理工藝,研究了投加2-MIB前后水中微生物種群的變化,建立了篩選分離2-MIB降解菌的方法.以生物膜生長成熟的活性炭連續處理含有較高濃度2-MIB水樣,然后從活性炭表面取樣,用2-MIB為惟一碳源的無機鹽培養基篩選和馴養,最后進行劃線分離,成功得到2-MIB降解菌種.通過16SrRNA分析,該菌株屬于假單胞菌屬(Pseudomonas spp.).

     

    Abstract: For the given water treatment,the features of the microbiological colonies before and after the addition of the 2-methylisoborneol(2-MIB) were studied,and a method for isolation of a bacterium capable of removing the 2-MIB from the water was proposed.The procedure of the method was as follows:the activated carbon,whose surface was covered by a mature microbiological membrane,was employed to continually treat the water with higher concentration of 2-MIB,and then the sample gained from the surface of the activated carbon was cultivated in the mineral salt liquid culture in which 2-MIB was the only carbon source;finally,one strain,which was identified as Pseudomonas spp.based on the data of 16S rRNA analysis,was isolated by plate streaks.

     

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