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產絮凝劑雙菌復合發酵體系的研究以及在水處理中的應用

Investigations on producing bioflocculant by double microorganisms and its application on water treatment

  • 摘要: 從土壤和活性污泥中篩選到三株絮凝劑產生菌,將其進行雙菌混合培養的正交試驗,構建出了比單一菌所產絮凝劑絮凝活性更高的復合菌群LH,通過16SrRNA序列分析,初步判定二菌分別為Micrococcus sp.和Paenibacill usvelaei菌.對其性質的初步研究發現,復合菌LH所產絮凝劑MBF-LH為胞外代謝產物,其熱穩定性好.LH產絮凝劑的最適碳源、最適培養溫度、最適pH值和通氣量分別為蔗糖、30℃、pH8和120r/min恒溫振蕩培養.培養基中添加KCl、CaSO4、K2HPO4能明顯提高發酵液絮凝率.絮凝劑MBF-LH處理廢水時具有用量少、絮凝率高的特點.

     

    Abstract: Three strains of bacteria which produce flocculating substances were isolated from activated sludge and soil.Every two strains of the bacteria among the 3 strains obtained above were selected to mix ferment by orthogonal experiment.The results showed that the combination of two strains called LH possessed the best flocculating activity.Identified on the base of 16SrRNA gene sequence,the two strains were pertained to Micrococcus sp.and Paenibacillus velaei.Though the preliminary study of the MBF-LH,it had been confirmed that MBF-LH is a kind of extracellular biopolymer and has a good thermostabilization.The best carbon source,optimal cultural temperature,initial pH value and rate of rotary shaker were saccharose,30℃,pH 8 and 120 r/min.Addition of KCl,CaSO4 and K2HPO4 to the culture medium could obviously increase the flocculating rate.When applied to the wastewater treatment,MBF-LH had some good properties such as low dosage and high efficiency.

     

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