Abstract: Based on the principle of two-point measurement method, a measuring technique for thermal para-meters of living tissue by using three-point temperature measurements was proposed. The corresponding theoretical model was established. The effects of thermal conductivity blood perfusion and volumetric heat capacity on the temperature variation were analyzed through numerical calculations. The results of sensitivity analysis show that the sensitivity coefficients of these thermal parameters are linearly independent each other, and thereby can be measured simultaneously by using this three-point method.