Abstract: The experiment indicates that under general temperature, acid and anaerobic condition, adding rational nutrient and electron supplier, with J13 bacteria from mining water adopting consequent culture in high density, pulp leaching in advance, microbe immobilization and enhance energy transmitting, which the leaching time is 3 days. The rate of metal leaching is Co 95.2%, Ni 97.5%, Mn 95.8%, Cu 81.2%. The same experiment was carried out on land magnetic ores, the leaching time is 9 days. Thus, a new method is set up that under the enforce bacteria of two ores are coupled and reduced to leach the valuable metals from oxide manganese ore.